Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins].

Abstract Text

A new two-dimensional electrophoresis is described. The first electrophoresis is performed in a 10 p. cent gel (1 X 110 mm) in the presence of 7 M urea; the migration in the second dimension proceeds on a 20 p. cent polyacrylamide sheet (160 X 110 X 1 mm) in the presence of sodium dodecyl sulphate. This system resolves most of the polypeptides obtained by treating a very small amount (about 100 mug) of proteins with cyanogene bromide. Macromolecules can thus be quickly and easily characterized.

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Citation:- Portier C, .Analysis of a small quatity of polypeptides by two-dimensional electrophoresis: application to characterization of proteins]. Biochimie 1975-;57()539-44 DOI:



Details of Journal

Journal Title - Biochimie

ISSN - 0300-9084

Volume - 57

Issue - 5

Publish date - 1975-

Language - fre

Country - FRANCE


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