BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping.

Abstract Text

Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical "contig" maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning approximately 10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual "BAC-HAPPY-mapping" to convert BAC landing data into BAC linkage contigs is possible.

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2016-09-07 19:14:21

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Citation:- Vu GT, Dear GT, Caligari GT, Wilkinson GT, .BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping. PloS one 2010-;5()e9089 DOI:10.1371/journal.pone.0009089

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Details of Journal

Journal Title - PloS one

ISSN - 1932-6203

Volume - 5

Issue - 2

Publish date - 2010-

Language - eng

Country - United States


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