Binpairs: utilization of Illumina paired-end information for improving efficiency of taxonomic binning of metagenomic sequences.

Abstract Text

Paired-end sequencing protocols, offered by next generation sequencing (NGS) platforms like Illumia, generate a pair of reads for every DNA fragment in a sample. Although this protocol has been utilized for several metagenomics studies, most taxonomic binning approaches classify each of the reads (forming a pair), independently. The present work explores some simple but effective strategies of utilizing pairing-information of Illumina short reads for improving the accuracy of taxonomic binning of metagenomic datasets. The strategies proposed can be used in conjunction with all genres of existing binning methods.

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2016-09-08 14:29:22

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Citation:- Dutta A, Tandon A, Mohammed A, Bose A, Mande A, .Binpairs: utilization of Illumina paired-end information for improving efficiency of taxonomic binning of metagenomic sequences. PloS one 2014-;9()e114814 DOI:10.1371/journal.pone.0114814

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Details of Journal

Journal Title - PloS one

ISSN - 1932-6203

Volume - 9

Issue - 12

Publish date - 2014-

Language - eng

Country - United States


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